Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B

Rotaviruses (RVs) are significant enteric pathogens of humans and animals. In March 2021, the University Kentucky Veterinary Diagnostic Laboratory (UKVDL) received a fecal sample from 1-week-old goat kid with diarrhea farm 5 additional diarrheic kids. The was tested negative for Bovine coronavirus , rotavirus Group A, E. coli K99+, Cryptosporidium parvum Salmonella spp by multiplex real-time PCR assays. Interestingly, novel Equine Rotavirus B (ERVB) in identified neonatal foals also watery Spring 2021. Once ERVB-specific assay became available, found positive RVB. Genome sequence caprine RVB obtained using shotgun metagenomic sequencing Illumina MiSeq. All eleven viral segments were sequenced either completely or partially. Genetic phylogenetic analysis VP7, VP4, VP6, VP1–VP3, NSP1- NSP5 genes indicated strain had genotype constellation G3-P[3]-I3- R3-C3-M3-A4-N3-T3-E3-H3. addition, showed highest nucleotide identity evolutionary relationship to ERVB previously strains. Given similar geographical location equine strains our study, these findings suggest possible common source infection.